rabbit polyclonal anti pedf  (Sino Biological)

Journal: iScience

Article Title: Chimpanzee adenovirus-mediated multiple gene therapy for age-related macular degeneration

doi: 10.1016/j.isci.2023.107939

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-PEDF , Sino Biological , Cat#11104-RP02.

Techniques: Virus, Recombinant, Transfection, Protease Inhibitor, Plasmid Preparation, Gel Extraction, Software

Journal: Molecules

Article Title: Proteome Analysis of Aflibercept Intervention in Experimental Central Retinal Vein Occlusion

doi: 10.3390/molecules27113360

Figure Lengend Snippet: Proteins that significantly changed in content following aflibercept intervention.

Article Snippet: After heating, slides remained in buffer for 15 min. Primary antibodies used for immunohistochemistry included a polyclonal rabbit anti-fibronectin antibody (A0245, Dako, Glostrup, Denmark) 1:1000, a primary polyclonal rabbit anti-galectin-3 antibody (MBS3211803, MyBioSource, San Diego, CA, USA) 1:200, a monoclonal mouse anti-neurofilament light polypeptide (M0762, Dako, Glostrup, Denmark) 1:100, a rat monoclonal anti-endoplasmin antibody (MBS439463, MyBioSource, San Diego, CA, USA) and a rabbit polyclonal anti-pigment epithelium derived factor (PEDF) antibody (MBS2027143, MyBioSource, San Diego, CA, USA).

Techniques:

Journal: Molecules

Article Title: Proteome Analysis of Aflibercept Intervention in Experimental Central Retinal Vein Occlusion

doi: 10.3390/molecules27113360

Figure Lengend Snippet: Volcano plot. Log 2 of the ratio aflibercept/NaCl is plotted on the x-axis. On the y-axis, −log p -value refers to the logarithmized p -value from the t -test used to test if a protein was significantly changed. Statistically significantly changed proteins are located above the horizontal line, which denotes a significance level of 0.05. Components of aflibercept are not included in the volcano plot. PEDF: pigment epithelium-derived factor. DNAJ7C: DnaJ homolog subfamily C member 7. AKAP8: A-kinase anchor protein 8. XAB2: Pre-mRNA-splicing factor SYF1. MRPS7: 28S ribosomal protein S7, mitochondrial. RPS18: 40S ribosomal protein S18. RER1: Protein RER1.

Article Snippet: After heating, slides remained in buffer for 15 min. Primary antibodies used for immunohistochemistry included a polyclonal rabbit anti-fibronectin antibody (A0245, Dako, Glostrup, Denmark) 1:1000, a primary polyclonal rabbit anti-galectin-3 antibody (MBS3211803, MyBioSource, San Diego, CA, USA) 1:200, a monoclonal mouse anti-neurofilament light polypeptide (M0762, Dako, Glostrup, Denmark) 1:100, a rat monoclonal anti-endoplasmin antibody (MBS439463, MyBioSource, San Diego, CA, USA) and a rabbit polyclonal anti-pigment epithelium derived factor (PEDF) antibody (MBS2027143, MyBioSource, San Diego, CA, USA).

Techniques: Derivative Assay

Journal: Molecules

Article Title: Proteome Analysis of Aflibercept Intervention in Experimental Central Retinal Vein Occlusion

doi: 10.3390/molecules27113360

Figure Lengend Snippet: Immunohistochemistry comparing CRVO + aflibercept vs. CRVO + NaCl. Proteome analysis showed a small downregulation of endoplasmin and PEDF, which was further explored with immunohistochemistry. Immunohistochemistry showed similar staining patterns of ( A – D ) endoplasmin and ( E – H ) PEDF regardless of aflibercept intervention. Thus, immunohistochemistry of retinas treated with aflibercept did not show major changes in regulation of the endoplasmin or PEDF in specific retinal layers. Scale bar = 100 µm. Reaction color: brown. Abbreviations: NFL: nerve fiber layer; GCL: ganglion cell layer; IPL: inner plexiform layer; INL: inner nuclear layer; OPL: outer plexiform layer; ONL: outer nuclear layer; PS: photoreceptor segments.

Article Snippet: After heating, slides remained in buffer for 15 min. Primary antibodies used for immunohistochemistry included a polyclonal rabbit anti-fibronectin antibody (A0245, Dako, Glostrup, Denmark) 1:1000, a primary polyclonal rabbit anti-galectin-3 antibody (MBS3211803, MyBioSource, San Diego, CA, USA) 1:200, a monoclonal mouse anti-neurofilament light polypeptide (M0762, Dako, Glostrup, Denmark) 1:100, a rat monoclonal anti-endoplasmin antibody (MBS439463, MyBioSource, San Diego, CA, USA) and a rabbit polyclonal anti-pigment epithelium derived factor (PEDF) antibody (MBS2027143, MyBioSource, San Diego, CA, USA).

Techniques: Immunohistochemistry, Staining

Journal: Molecules

Article Title: Proteome Analysis of Aflibercept Intervention in Experimental Central Retinal Vein Occlusion

doi: 10.3390/molecules27113360

Figure Lengend Snippet: Western blots comparing CRVO + Eylea vs. CRVO + NaCl. ( A – B ) Western blotting was performed in two replicates. ( C – D ) Averages of quantitative data from the two replicates. The changes observed by mass spectrometry in endoplasmin and PEDF following aflibercept intervention were too slight to be confirmed by Western blotting. ( E – G ) Horizontal lines of the plots denote the means of the quantitative data. Standard deviations were larger in data obtained with Western blotting compared with quantitative data obtained through proteomic analysis. Standard deviations of endoplasmin quantification with mass spectrometry and Western blotting were 0.14 and 0.43, respectively. Standard deviations of PEDF quantification with mass spectrometry and Western blotting were 0.23 and 0.76, respectively.

Article Snippet: After heating, slides remained in buffer for 15 min. Primary antibodies used for immunohistochemistry included a polyclonal rabbit anti-fibronectin antibody (A0245, Dako, Glostrup, Denmark) 1:1000, a primary polyclonal rabbit anti-galectin-3 antibody (MBS3211803, MyBioSource, San Diego, CA, USA) 1:200, a monoclonal mouse anti-neurofilament light polypeptide (M0762, Dako, Glostrup, Denmark) 1:100, a rat monoclonal anti-endoplasmin antibody (MBS439463, MyBioSource, San Diego, CA, USA) and a rabbit polyclonal anti-pigment epithelium derived factor (PEDF) antibody (MBS2027143, MyBioSource, San Diego, CA, USA).

Techniques: Western Blot, Mass Spectrometry

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Secreted PEDF modulates fibroblast collagen synthesis through M1 macrophage polarization under expanded condition.

doi: 10.1016/j.biopha.2021.111951

Figure Lengend Snippet: Fig. 1. Increased expression of PEDF in expanded human and mouse epidermis. A. Immunofluorescence staining for PEDF (green) and DAPI (blue) in the epithelial cells of human expanded skin (n = 9) and control skin (n = 6, ×40 magnification); B. Quantification of PEDF-positive cells per field in human skin (n = 6, mean ± SEM; **P < 0.01); C. Immunofluorescence staining for PEDF (green) and DAPI (blue) in the epithelial cells in mouse expanded skin and control skin (n = 6, ×40 magnification); D. Quantification of PEDF-positive cells per field in mouse skin (n = 6, mean ± SEM; ***P < 0.001). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: After deparaffinized and rehydrated in alcohol series, skin sections were kept in a sodium citrate solution and heated in a microwave oven to 95 °C for 20 min before being incubated with a rabbit anti-mouse PEDF primary polyclonal antibody at a 1:30 dilution (Boster Bio-Engineering, Hubei Province, China) at 4 °C overnight.

Techniques: Expressing, Immunofluorescence, Staining, Control

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Secreted PEDF modulates fibroblast collagen synthesis through M1 macrophage polarization under expanded condition.

doi: 10.1016/j.biopha.2021.111951

Figure Lengend Snippet: Fig. 3. PEDF accumulated in the subcutaneous exudates of a rat skin expansion model. The quantity of PEDF protein in the subcutaneous exudates obtained at indicated times in expanded and control rats were determined by ELISA assays (n = 6, mean ± SEM; ***P < 0.001).

Article Snippet: After deparaffinized and rehydrated in alcohol series, skin sections were kept in a sodium citrate solution and heated in a microwave oven to 95 °C for 20 min before being incubated with a rabbit anti-mouse PEDF primary polyclonal antibody at a 1:30 dilution (Boster Bio-Engineering, Hubei Province, China) at 4 °C overnight.

Techniques: Control, Enzyme-linked Immunosorbent Assay

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Secreted PEDF modulates fibroblast collagen synthesis through M1 macrophage polarization under expanded condition.

doi: 10.1016/j.biopha.2021.111951

Figure Lengend Snippet: Fig. 2. Skin expansion resulted in dermal thinning and PEDF up-regulation in a mouse skin expansion model. A. H&E staining at the 3rd week after expansion, showing thinner dermis in the expanded skin (×40 magnification); B. Quantification of dermal thickness between expanded and control skin (n = 6, mean ± SEM; **P < 0.01); C. The mRNA expression levels of PEDF in skin obtained at the 3rd week compared with the control skin (n = 6, mean

Article Snippet: After deparaffinized and rehydrated in alcohol series, skin sections were kept in a sodium citrate solution and heated in a microwave oven to 95 °C for 20 min before being incubated with a rabbit anti-mouse PEDF primary polyclonal antibody at a 1:30 dilution (Boster Bio-Engineering, Hubei Province, China) at 4 °C overnight.

Techniques: Staining, Control, Expressing

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Secreted PEDF modulates fibroblast collagen synthesis through M1 macrophage polarization under expanded condition.

doi: 10.1016/j.biopha.2021.111951

Figure Lengend Snippet: Fig. 5. In vivo injection of PEDF promoted M1 macrophage polarization. A. A larger number of CD68+/iNOS+ double-positive macrophages were detected in the PEDF injection group compared with PBS treatment. In contrast to PEDF injection, LR antibody injection decreased the number of CD68+/iNOS+ double-positive macrophages; B. Quantification of M1 macrophages in differently treated skin (mean ± SEM; **P < 0.01，*P < 0.05).

Article Snippet: After deparaffinized and rehydrated in alcohol series, skin sections were kept in a sodium citrate solution and heated in a microwave oven to 95 °C for 20 min before being incubated with a rabbit anti-mouse PEDF primary polyclonal antibody at a 1:30 dilution (Boster Bio-Engineering, Hubei Province, China) at 4 °C overnight.

Techniques: In Vivo, Injection

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Secreted PEDF modulates fibroblast collagen synthesis through M1 macrophage polarization under expanded condition.

doi: 10.1016/j.biopha.2021.111951

Figure Lengend Snippet: Fig. 4. Blockage of PEDF receptor rescued dermal thinning in vivo. A. HE staining results showed that recombinant PEDF protein injection decreased the thickness of expanded dermis, while LR blockage rescued dermal thinning of expanded skin; B. Quantification of dermal thickness between differently treated skin (n = 6, mean ± SEM; ***P < 0.001，*P < 0.05).

Article Snippet: After deparaffinized and rehydrated in alcohol series, skin sections were kept in a sodium citrate solution and heated in a microwave oven to 95 °C for 20 min before being incubated with a rabbit anti-mouse PEDF primary polyclonal antibody at a 1:30 dilution (Boster Bio-Engineering, Hubei Province, China) at 4 °C overnight.

Techniques: In Vivo, Staining, Recombinant, Injection

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Secreted PEDF modulates fibroblast collagen synthesis through M1 macrophage polarization under expanded condition.

doi: 10.1016/j.biopha.2021.111951

Figure Lengend Snippet: Fig. 7. Hypoxia induced PEDF expression in HaCaT cells. The mRNA expression levels of PEDF in HaCaT cells under normoxic and hypoxic condi tions were determined by RT-qPCR (mean ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001).

Article Snippet: After deparaffinized and rehydrated in alcohol series, skin sections were kept in a sodium citrate solution and heated in a microwave oven to 95 °C for 20 min before being incubated with a rabbit anti-mouse PEDF primary polyclonal antibody at a 1:30 dilution (Boster Bio-Engineering, Hubei Province, China) at 4 °C overnight.

Techniques: Expressing, Quantitative RT-PCR

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Secreted PEDF modulates fibroblast collagen synthesis through M1 macrophage polarization under expanded condition.

doi: 10.1016/j.biopha.2021.111951

Figure Lengend Snippet: Fig. 8. PEDF promoted macrophages to polarize towards the M1 subtype under hypoxic conditions. A. FACs analysis of Raw264.7 single-cell sus pensions prepared after treating with PEDF under hypoxic conditions. CD11c+ cells indicate M1 macrophages. B. Quantification of CD11c+ cells under hypoxia (mean ± SEM; **P < 0.01). C. FACS analysis of Raw264.7 single-cell suspensions prepared after treating with PEDF under hypoxic conditions. CD206+ cells indicate M2 macro phages. D. Quantification of CD206+ cells under hypoxic conditions. The mRNA expression of M1 marker genes (E. iNOS, F. TNF-α) and M2 marker genes (G. Arg-1, H. Ym-1) determined by RT-qPCR and normalized to GAPDH mRNA expression. (mean ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001).

Article Snippet: After deparaffinized and rehydrated in alcohol series, skin sections were kept in a sodium citrate solution and heated in a microwave oven to 95 °C for 20 min before being incubated with a rabbit anti-mouse PEDF primary polyclonal antibody at a 1:30 dilution (Boster Bio-Engineering, Hubei Province, China) at 4 °C overnight.

Techniques: Expressing, Marker, Quantitative RT-PCR

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Secreted PEDF modulates fibroblast collagen synthesis through M1 macrophage polarization under expanded condition.

doi: 10.1016/j.biopha.2021.111951

Figure Lengend Snippet: Fig. 9. PEDF hindered collagen syn thesis in a macrophage-mediated manner under hypoxic conditions. A, B. Relative mRNA expression of COLI (A) and COLIII (B) in fibroblasts after co-culture with PEDF treated macro phages determined by RT-qPCR (mean

Article Snippet: After deparaffinized and rehydrated in alcohol series, skin sections were kept in a sodium citrate solution and heated in a microwave oven to 95 °C for 20 min before being incubated with a rabbit anti-mouse PEDF primary polyclonal antibody at a 1:30 dilution (Boster Bio-Engineering, Hubei Province, China) at 4 °C overnight.

Techniques: Expressing, Co-Culture Assay, Quantitative RT-PCR

Journal: International journal of molecular medicine

Article Title: MicroRNA‑126 suppresses the proliferation and migration of endothelial cells in experimental diabetic retinopathy by targeting polo‑like kinase 4.

doi: 10.3892/ijmm.2020.4775

Figure Lengend Snippet: Figure 3. Intraocular delivery of miR‑126 alleviates experimental diabetic retinopathy in diabetic rats. (A) H&E staining of retinal paraffin‑embedded sections (magnification, x200). ILM, inner limiting membrane; GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer; PEL, pigment epithelial layer. (B) The prepared retinal vasculature by trypsin digest (magnification, x400). Endothelial cells are indicated by white arrows, pericytes are indicated by black arrows and acellular capillaries are indicated by triangles. (C) Retinal immunofluorescence staining for CD34. (D) The protein contents of VEGF and PEDF in the rat retina. VEGF, vascular endothelial growth factor; PEDF, pigment epithelium‑derived factor; NDM+NC, non‑diabetic rats injected with lenti‑NC; DM+NC, diabetic rats injected with lenti‑NC; DM+miR‑126, diabetic rats injected with lenti‑miR‑126. *P<0.05; ***P<0.001; ****P<0.0001.

Article Snippet: After blocking with 5% non-fat dried milk solution at room temperature for 2 h, the membranes were first incubated overnight at 4 ̊C with polyclonal rabbit anti‐vascular endothelial growth factor (VEGF) antibody (A12303, ABclonal; 1:3,000), polyclonal rabbit anti-pigment epithelium-derived factor (PEDF) antibody (A11782, ABclonal; 1:3,000), polyclonal rabbit anti-PLK4 antibody (12952-1-AP, ProteinTech Group, Inc.; 1:1,000) or monoclonal mouse anti‐β-tubulin antibody (CW0098, ComWin Biotech; 1:5,000), and then with appropriate peroxidase-labeled secondary anti-rabbit or anti-mouse antibodies (RM3002, or RM3001, Beijing Ray Antibody Biotech; 1:3,000) at room temperature for 1 h. chemiluminescent signals were visualized using Ncm-EcL Ultra (New cell & Molecular Biotech co., Ltd.), while ImageJ software was used to determine the gray strip values.

Techniques: Staining, Membrane, Immunofluorescence, Injection